Human Kallikrein 3 ELISA Kit

Prostate-specific antigen (PSA), also known as kallikrein 3, seminin, semenogelase, gamma-seminoprotein and P-30 antigen, is a 34 kDa glycoprotein manufactured almost exclusively by the prostate gland. PSA is produced for the ejaculate where it liquefies the semen in the seminal coagulum and allows sperm to swim freely. PSA is believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is a serine protease enzyme, and is located on chromosome 19 (19q13). ScienCell's human Kallikrein 3 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Kallikrein 3-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human Kallikrein 3 in the sample that is captured on the strips.