Human HIVEP2(Transcription Factor HIVEP2) ELISA Kit

This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation Assay Type: Sandwich. Sensitivity: 0.102 ng/mL. Standard: 20 ng/mL. Detection range: 0.32-20 ng/mL. Sample type: serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h. Research Field: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIVEP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIVEP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIVEP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIVEP2 in the samples is then determined by comparing the OD of the samples to the standard curve.