Human FABP4 (Fatty Acid Binding Protein 4, Adipocyte) Microsample ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FABP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FABP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FABP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FABP4 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 0.78-50 ng/mL. Sensitivity: 0.2 ng/mL. Assay Type: Sandwich. Standard: 50 ng/mL. Sample Type: sSerum, plasma, tissue homogenates and other biological fluids. Assay duration: 3.5h. Protein Function: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus [The Uniprot Consortium]