Human ET-1 (Endothelin1) ELISA Kit

Human ET-1 (Endothelin1) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ELK-ELK2444.48 48 wells - -

10 - 14 business days*

365.00€
ELK-ELK2444.96 96 wells - -

10 - 14 business days*

522.00€
 
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate... more
Product information "Human ET-1 (Endothelin1) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 7.82-500 pg/mL. Sensitivity: 2.71 pg/mL. Assay Type: Sandwich. Standard: 500 pg/mL. Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay duration: 3.5h
Keywords: EDN1
Supplier: ELK Biotechnology
Supplier-Nr: ELK2444

Properties

Application: ELISA
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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