Human CRH(Corticotropin Releasing Hormone) ELISA Kit

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity) Assay Type: Competitive Inhibition. Sensitivity: 0.13 ng/mL. Standard: 20 ng/mL. Detection range: 0.32-20 ng/mL. Sample type: serum, plasma and other biological fluids. Assay length: 2.5h. Research Field: Endocrinology,Cardiovascular biology,Neuro science,Hormone metabolism,Urology,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.