Human CCL4/MIP-1 beta ELISA Kit

Human chemokine (C-C motif) ligand 4 (CCL4) is a protein that is encoded by the CCL4 gene. Also known as Macrophage inflammatory protein-1beta (MIP-1beta), it is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Modi et al. (1991) assigned the SCYA4 gene to a slightly more distal location than had Irving et al. (1990): 17q21-q23 rather than 17q11-q21. ScienCell's human CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human CCL4-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human CCL4 captured in strips.