Human ARHGDIb (Rho GDP Dissociation Inhibitor Beta) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARHGDIb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARHGDIb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARHGDIb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARHGDIb in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:7512369, PubMed:8356058). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133) [The Uniprot Consortium]