HSP27 Colorimetric Cell-Based BioAssay(TM) ELISA Kit

The HSP27 Colorimetric Cell-Based ELISA Kit can be used for measuring the amount of HSP27 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators haev on HSP27. Sensitivity: >5000cells/well, Kit Components: 472291A: Microtiter Strips, 1x96 wells, 472291B: TBS, 10X, 1x24ml, 472291C: Quenching Buffer, 1x25ml, 472291D: Blocking Buffer, 1x50ml, 472291E: Wash Buffer, 15X, 1x50ml, *472291F: HSP Pab, 100X, 1x60ul, *472291G: GAPDH Mab, 100X, 1x60ul, 472291H: IgG Pab (HRP), 1x6ml, 472291J: IgG Mab (HRP), 1x6ml, 472291K: Primary Antibody Diluent, 1x12ml, 472291L: Substrate, 1x12ml, 472291M: Stop Solution (2N Sulfuric Acid), 1x12ml, 472291N: Crystal Violet Solution, 1x6ml, *472291P: SDS Solution, 1x24ml, Storage and Stability: Store all components at 4°C. Reconstituted *472291F and *472291G are stable for 2 weeks at 4°C.. *472291P should be stored at RT or wrmed up to RT if stored at 4ºC. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.