Hepatitis E Virus, IgG (HEV), ELISA Kit, BioAssay(TM)

Intended Use:, Hepatitis E Virus, IgG (HEV), ELISA Kit, BioAssay(TM) is an in vitro enzyme immunoassay for the detection of Anti-HEV antibodies IgG in human serum or plasma. It is for diagnosis of early infection and epidemic survey. Principle of the Test: This kit uses indirect ELISA method to detect anti-HEV antibodies IgG in serum or plasma. The purified activated recombinant HEV antigen (ORF2, ORF3) is coated on the multi-wells plate. The HRP conjugated mouse anti-human IgG (r chain) monoclonal antibody serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450nm indicates the presence or absence of Anti-HEV antibodies IgG in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HEV infection. Recommended Instruments: 8x12 wells plate reader and washer, Kit Components: H1927-01W1: Antigen Coated Microtiter Strips, 96 wells, H1927-01W2: Sample Diluent, 12ml, H1927-01W3: Negative Control Serum, 1ml, H1927-01W4: Positive Control Serum, 1ml, H1927-01W5: Concentrated Wash Buffer (20X), 50ml, H1927-01W6: Enzyme Conjugant, 12ml, H1927-01W7: Substrate A, 6ml, H1927-01W8: Substrate B, 6ml, H1927-01W9: Stop Solution, , H2SO4, 6ml, Also Included: Plastic Bag, Seal Paper, Kit Manual , Storage and Stability:, Store the kit at 4°C. Stable for 6 months after receipt.