HBEGF BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human HBEGF in cell culture supernates, serum and plasma (heparin, EDTA). HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults. , Assay Range:, 15.6pg/ml-1000pg/ml, Sensitivity: < 10 pg/ml , Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: Q99075, Entrez Gene ID: 1839, Kit Components: 96-well plate pre-coated with anti-HBEGF, Human, Lyophilized recombinant HBEGF, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.