Growth Regulated Oncogene Beta (GROb) BioAssay(TM) ELISA Kit (Human)

Growth Regulated Oncogene Beta (GROb) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of GROb in human serum, plasma and other biological fluids. Detection Range: 15.6-1,000pg/ml, Sensitivity: 6.1pg/ml, Intra-Assay: CV?10%, Inter-Assay: CV?12%, Kit Components: *025516A: Microtiter Plate, 1x96wells, Pre-coated, ready to use, *025516B: Standard, 2x1vial , 025516C: Standard Diluent, 1x20ml, *025516D: Detection Reagent A, 1x120ul , *025516E: Detection Reagent B, 1x120ul , 025516F: Assay Diluent A, 1x12ml, 025516G: Assay Diluent B, 1x12ml, 025516H: TMB Substrate, 1x9ml, 025516K: Stop Solution, 1x6ml, 025516L: Wash Buffer, 30X, 1x20ml , Precaution: The Stop Solution (025516K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025516A, *025516B, *025516D and *025516E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul 025516H: TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul 025516K: Stop Solution. Read at 450nm immediately.