Fibulin-3/EFEMP1 BioAssay(TM) ELISA Kit (Human)

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminal fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human Fibulin-3/EFEMP1. Sensitivity: <10pg/ml, Range: 312pg/ml-20,000pg/ml, Specificity: Recognizes natural and recombinant Human Fibulin-3/EFEMP1. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human Fibulin-3/EFEMP1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A polyclonal antibody from goat specific for Fibulin-3/EFEMP1 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: Q18-F493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibulin-3/EFEMP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Fibulin-3/EFEMP1 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human EFEMP1 antibody, 1x plate, Lyophilized recombinant Human EFEMP1 standard, 2x20ng/tube, Biotinylated anti-Human EFEMP1 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.