FGF21 BioAssay(TM) ELISA Kit, Human

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Uniprot ID: Q9NSA1, Entrez Gene ID: 26291, Intended Use: Fibroblast Growth Factor 21 (FGF21) BioAssay(TM) ELISA Kit, Human is a sandwich ELISA for quantitative detection of human FGF21 in cell culture supernates, serum and plasma (heparin, EDTA). Range: 31.2-2000pg/ml, Sensitivity: <10pg/ml, Specificity: Natural and recombinant human FGF21, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 143831A: Microtiter Strips, 1x96 wells, *143831B: FGF21 Standard, 2x1 vial, 143831C: Anti-human FGF21 (Biotin), 1x130ul, USB Cat No Kit Component 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample Diluent Buffer, 1x30ml, 143698: Antibody Diluent Buffer, 1x12ml, 143699: ABC Diluent Buffer, 1x12ml, 143700: TMB Color Developing Agent, 1x10ml, 143701: TMB Stop Solution, 2N H2SO4, 1x10ml, Assay Summary: 1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. , 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS., 3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes., 5. Add TMB stop solution and read. Storage and Stability: Store *143831B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.