FASL BioAssay(TM) ELISA Kit, Human

Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7. Intended Use: For quantitative detection of human FASL in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Range: 15.6-1000pg/ml, Sensitivity: < 2pg/ml, Specificity: Natural and recombinant human FASL, Crossreactivity: No detectable crossreactivity with other relevant proteins, Kit Components: 143829A: Microtiter Strip, 1x96 wells, 143829B: FASL Standard, 2x10ng, 143829C: Anti-human FASL (Biotin), 130ul, USB Cat No Kit Component, 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution), 143697: Sample diluent buffer, 1x30ml, 143698: Antibody diluent buffer, 1x12ml, 143699: ABC diluent buffer, 1x12ml, 143700: TMB color developing agent, 1x10ml, 143701: TMB stop solution, 2N H2SO4, 1x10ml, Storage and Stability: Store *143829B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Add 100ul of samples and standards and incubate the plate at 37°C for 90 min. Do not wash., 2. Add 100ul biotinylated antibody and incubate the plate at 37°C for 60 min. Wash plate 3 times with 0.01M TBS or PBS., 3. Add ABC working solution and incubate the plate at 37°C for 30min. Wash plate 5 times with 0.01M TBS or PBS., 4. Add TMB color developing agent and incubate the plate at 37°C in dark for 20-25 min., 5. Add 100ul TMB stop solution and read.