EasyStep Human sTFR (Soluble Transferrin Receptor) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR, and the Human sTFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human sTFR. After TMB substrate solution is added, only those wells that contain Human sTFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 78.13-5000 ng/mL. Sensitivity: 17.55 ng/mL. Assay Type: Sandwich. Standard: 5000 ng/mL. Sample Type: sSerum, plasma. Assay duration: 1.5h. Protein Function: Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse [The Uniprot Consortium]