EasyStep Human APOB100 (Apolipoprotein B100) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB100, and the Human APOB100 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human APOB100. After TMB substrate solution is added, only those wells that contain Human APOB100 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.Detection Range: 0.16-10 µg/mL. Sensitivity: 41 ng/mL. Assay Type: Sandwich. Standard: 10 µg/mL. Sample Type: sSerum, plasma. Assay duration: 1.5h. Protein Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor [The Uniprot Consortium]