Cyclic GMP ELISA Kit

Cyclic GMP ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay581021-96S 96 so-wells (96 solid wells) -

6 - 10 business days*

338.00€
Cay581021-96 96 st-wells (96 strip wells) -

6 - 10 business days*

338.00€
Cay581021-480 480 st-wells (480 strip wells) -

6 - 10 business days*

1,337.00€
Cay581021-480S 480 so-wells (480 solid wells) -

6 - 10 business days*

1,337.00€
 
The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP... more
Product information "Cyclic GMP ELISA Kit"
The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2' hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions. Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106 cells). Synonyms: cGMP EIA Kit, Guanosine 3',5'-cyclic mononucleotide, Guanosine 3',5'-cyclic monophosphate, Monosodium-GMP. Assay Range: 0.23-30 pmol/ml. Sensitivity: 80% B/B0: 1 pmol/ml. Mid-point: 50% B/B0: 3-6 pmol/ml. Cross Reactivity: (Non-Acetylated cGMP) 9%, (Non-Acetylated Dibutyryl cGMP) 0.8%, (Non-Acetylated AMP) <0.01%, (Non-Acetylated ATP) <0.01%, (Non-Acetylated cAMP) <0.01%, (Non-Acetylated 8-bromo-cGMP) <0.01%, (Acetylated cGMP) 100%, (Acetylated cAMP) 0.05%, (Acetylated adenosine) <0.01%, (Acetylated AMP) <0.01%, (Acetylated cytidine) <0.01%, (Acetylated guanosine) <0.01%, (Acetylated uridine) <0.01%. Origin: Animal/Bovine, Animal/Eel, Animal/Mouse, Animal/Rabbit.
Keywords: cGMP EIA Kit, Guanosine 3',5'-cyclic mononucleotide, Guanosine 3',5'-cyclic monophosphate, Monosodium-GMP
Supplier: Cayman Chemical
Supplier-Nr: 581021

Properties

Application: ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H226, H301, H302, H311, H313, H314, H315, H316, H317, H318, H319, H332, H335, H351, H361, H402
P Phrases: P201, P202, P210, P233, P240, P241, P242, P243, P260, P261, P264, P270, P271, P272, P273, P280, P301+310, P301+330+331, P302+352, P303+361+353, P304+340, P305+351+338, P308+313, P310, P312, P321, P330, P332+313, P333+313, P337+313, P361+364, P362+364, P363, P370+378, P403+233,
Caution
Our products are for laboratory research use only: Not for administration to humans!
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