Cross Linked C-Telopeptide of Type III Collagen (CTXIII) BioAssay(TM) ELISA Kit (Human)

The Cross Linked C-Telopeptide of Type III Collagen (CTXIII) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of CTXIII in human serum, plasma and other biological fluids. Detection Range: 123.5-10,000pg/ml, Sensitivity: 46.8pg/ml, Precision: Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit Components: *024471A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated, ready to use., *024471B: Standard, 2x1vial , 024471C: Standard Diluent, 1x10ml or 1x20ml, *024471D: Detection Reagent A, 1x1vial , *024471E: Detection Reagent B, 1x60ul or 1x120ul , 024471F: Assay Diluent A, 1x6ml or 1x12ml, 024471G: Assay Diluent B, 1x6ml or 1x12ml, 024471H: TMB Substrate, 1x4.5ml or 1x9ml, 024471K: Stop Solution, 1x3ml or 1x6ml, 024471L: Wash Buffer, 30X, 1x10ml or 1x20ml, 024471M: Detection Reagent A Diluent for 024471D, 1x300ul, Precaution: The Stop Solution (024471K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024471A, *024471B, *024471D and *024471E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C., 3. Aspirate and wash 3 times., 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 5. Aspirate and wash 5 times., 6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C., 7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.