Complement 1 Inhibitor (C1INH) BioAssay(TM) ELISA Kit (Human)

Complement 1 Inhibitor (C1INH) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of C1INH in human serum, plasma and other biological fluids. Detection Range: 0.78-50ng/ml, Sensitivity: 0.31ng/ml, Intra-Assay: CV<10%, Inter-Assay: CV<12%, Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1INH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C1INH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1INH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C1INH in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *024327A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *024327B: Standard, 2x1vial , 024327C: Standard Diluent, 1x20ml, *024327D: Detection Reagent A (green), 1x120ul , *024327E: Detection Reagent B (red), 1x120ul , 024327F: Assay Diluent A, 1x12ml, 024327G: Assay Diluent B, 1x12ml, 024327H: TMB Substrate, 1x9ml, *024327J: Positive Control, 17.43ng/ml, 1x1vial, 024327K: Stop Solution, 1x6ml, 024327L: Wash Buffer, 30X, 1x20ml , Precaution: The Stop Solution (024327K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024327A, *024327B, *024327D, *024327E and *024327J at -20°C. After reconstitution of *024327J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 024327J - Positive Control: Intended Use:, The control vials are from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vials contain human NEFH in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. Expected Value: 17.43ng/ml, Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.