Chemerin BioAssay(TM) ELISA Kit (Human)

This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein. Sample Type: Serum, plasma, tissue homogenates, Intended Use: For the quantitative determination of human chemerin concentrations in serum, plasma, tissue homogenates. Sensitivity: 7.8ng/ml, Range: 31.2-2000ng/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of human chemerin. No significant cross-reactivity or interference between human chemerin and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for chemerin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any chemerin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for chemerin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of chemerin bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells, Standard (Freeze dried), 2x vials, Biotin-antibody (100X), 1x120ul, HRP-avidin (100X), 1x120ul, Biotin-antibody Diluent, 1x15ml, HRP-avidin Diluent, 1x15ml, Sample Diluent, 1x50ml, Wash Buffer (25X), 1x20ml, TMB Substrate, 1x10ml, Stop Solution, 1x10ml, Adhesive Strip (For 96 wells), 4x strips, Instruction manual, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.