CD244/2B4 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human CD244/2B4 in cell culture supernates, serum and plasma (heparin, EDTA). CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2. , Assay Range:, 62.5pg/ml-4000pg/ml, Sensitivity: < 10 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: Q9BZW8, Entrez Gene ID: 51744, Kit Components: 96-well plate pre-coated with anti-CD244/2B4, Human, Lyophilized recombinant CD244/2B4, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.