Bovine LPO (Lactoperoxidase) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bovine LPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bovine LPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bovine LPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bovine LPO in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Heme-containing oxidoreductase which catalyzes the conversion of thiocyanate (SCN(-)) into antimicrobial agent hypothiocyanous acid (OSCN(-)) in the presence of hydrogen peroxide (H2O2) (Probable) (PubMed:5338806). Also involved in the conversion of iodide (I(-)) into hypoiodite (IO(-)) in the presence of H2O2 (Probable) (PubMed:354515). Responsible for the inactivation of a wide range of micro-organisms and hence, important component of defense mechanism (PubMed:354515, PubMed:5338806). The lactoperoxidase-SCN(-)-H2O2 system shows antibacterial properties against some streptococci strains (PubMed:5338806). The lactoperoxidase-I(-)-H2O2 system shows antibacterial properties against E.coli (PubMed:354515). May protect the udder from infection and may promote growth in newborns (By similarity). May be implicated in airway host defense against infection (By similarity). May contribute to maintaining an appropriate H2O2 cellular level, therefore protecting cells from H2O2-caused injuries and inflammation (By similarity) [The Uniprot Consortium]