BMP-6 BioAssay(TM) ELISA Kit (Rat)

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. Sample Type: Cell culture supernates, serum, bone tissue, tissue. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rat BMP-6. Sensitivity: <10pg/ml, Range: 156pg/ml-10,000pg/ml, Specificity: Recognizes natural and recombinant Rat BMP-6. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rat BMP-6 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A a monoclonal antibody from mouse specific for BMP-6 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S368-H506) and test samples are added to the wells, a biotinylated detection a biotinylated polyclonal antibody from goat specific for BMP-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BMP-6 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rat Bmp6 antibody, 1x plate, Lyophilized recombinant Rat Bmp6 standard, 2x10ng/tube, Biotinylated anti-Rat Bmp6 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.