Bispecific Mesothelin:CD3 Bridging Chemiluminescence ELISA Kit

The Bispecific Mesothelin:CD3 Bridging Chemiluminescence ELISA Kit is an ELISA designed to analyze the ability of Bispecific Antibodies (BsAbs) to bridge Mesothelin (also known as MSLN, MPF, SMRP) and CD3 (cluster of differentiation 3) for screening and profiling applications. This assay kit can determine if an anti-Mesothelin-anti-CD3 bispecific antibody binds to both targets simultaneously and bridges Mesothelin to CD3. The Bispecific Mesothelin:CD3 Bridging Chemiluminescence ELISA Assay Kit comes with enough recombinant human Mesothelin (amino acids 296-580), CD3-Containing Detection Reagent, and assay buffer for 100 enzyme reactions. This kit also includes Anti-Mesothelin-Anti-CD3 Bispecific Antibody as a positive control. Figure 1: Bispecific Mesothelin:CD3 Bridging Chemiluminescence ELISA Assay Kit principle. A 96-well plate is coated with Mesothelin protein. After coating, an anti-Mesothelin-anti-CD3 bispecific antibody is added in an optimized assay buffer. The plate is washed to remove the unbound antibody. The plate is then incubated with CD3-Containing Detection Reagent. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the bridging efficacy. Need us to run inhibitor screens or profile your bispecific antibodies towards CD3 and mesothelin? Check out our Immunotherapy Biochemical Screening Services.