Beta-EP (Beta-Endorphin) BioAssay(TM) ELISA Kit (Sheep)

The Beta-EP (Beta-Endorphin) BioAssay(TM) ELISA Kit (Sheep) is a quantitative competitive assay for the detection of beta-EP in serum, plasma, tissue homogenates and other biological fluids, Sensitivity: 9.375pg/ml, Detection Range: 15.625-1000pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of beta-EP. No significant cross-reactivity or interference between beta-EP and analogues is observed. Precision: Intra-Assay: CV<8%, Inter-Assay: CV<10%, Test Principle: The microtiter plate provided in this kit has been pre-coated with beta-EP. During the reaction, beta-EP in the sample or standard competes with a fixed amount of beta-EP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to beta-EP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of beta-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: Micro ELISA Plate(Dismountable), 8×12, Lyophilized Standard, 2x vial, Sample / Standard dilution buffer, 1x20ml, Biotin-detection antibody (Concentrated), 1x60ul, Antibody dilution buffer, 1x10ml, HRP-Streptavidin Conjugate(SABC), 1x120ul, SABC dilution buffer, 1x10ml, TMB substrate, 1x10ml, Stop solution, 1x10ml, Wash buffer (25X), 1x30ml, Plate Sealer, 1x5pieces, Storage and Stability: Store unopened kit at 4ºC, store at -20ºC once opened. Store unopened standard at 4°C, once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.