Anti-Hepatitis B Virus E Antigen (anti-HBeAg) ELISA Kit, BioAssay(TM) (Human)

The Anti-Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of anti-HBe in human serum or plasma. Principle of the Assay: The purified Anti-HBe is coated on the solid phase of multi-wells. Serum sample, recombinant HBeAg and horseradish peroxidase labeled with Anti-HBe (conjugated) are added to coated wells, and form a competitive combination. After incubation, if Anti-HBe content is high in the sample, a complex of HBeAg-Anti-HBe-Anti-HBe labeled with HRP will form. The wells are washed to remove the complex, and incubated with substrate (TMB), subsequently producing no color change or little color change. If Anti-HBe is not present in the sample, there will be significant color change. The test is sensitive, reproducible and easy to operate. Kit Components: H1910-40J1: Microtiter Plate, 1x96wells, H1910-40J2: Enzyme Conjugate, 1x6ml, H1910-40J3: Positive Control Serum, 1x500ul, H1910-40J4: Negative Control Serum, 1x500ul, H1910-40J5: Wash Buffer (20X), 1x30ml, H1910-40J6: Substrate A, 1x6ml, H1910-40J7: Substrate B, 1x6ml, H1910-40J8: Stop Solution, 1x6ml, H1910-40J9: Neutralization Reagent, 1x6ml, Interpretation of Results: Cut-off Value (COV) Calculation:, COV = average OD450 of negative controls X 0.5 , Positive: OD450 of sample COV, Invalid: If the OD of negative control is below or equal to 1.000, the result is invalid. In any event, repeat the test. If the problem persists, contact technical support. Performance Characteristics: Sensitivity: 4NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD <0.600, Specificity: The average OD of 20 normal negative smples is >2.00, Precision: %CV <20% (n=10), Storage and Stability: Store all kit components at 4°C. Kit is stable for 6 months.