Activin A BioAssay(TM) ELISA Kit (Porcine)

Activin A is a homodimer of 14kD beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this BioAssay(TM) kit is recombinant Activin A, which is composed of two single chains of 116aa with the molecular mass of 26kD. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and saliva. Intended Use: Sandwich High Sensitivity ELISA BioAssay(TM) kit for Quantitative Detection of Porcine Activin A. Sensitivity: <12pg/ml, Range: 15.6pg/ml-1000pg/ml, Specificity: Recognizes natural and recombinant porcine Activin A. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine Activin A BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine Activin A has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Porcine Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine Activin A amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine INHBA antibody, 1x plate, Lyophilized recombinant Porcine INHBA standard, 2x10ng/tube, Biotinylated anti-Porcine INHBA antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.