ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Human Therapeutic IgG4 ELISA Kit
Human Therapeutic IgG4 ELISA Kit

Item number: Cay500920-96

Cayman's Human Therapeutic IgG4 ELISA Kit is an immunometric (i.e. sandwich) assay that can be used for the quantification of human IgG4 in monkey, rodent, or rabbit plasma and serum without prior sample purification. The standard curve spans the range of 7.8-500 ng/ml, with a lower limit of quantification (LLOQ) of...
Keywords: Human IgG4 Immunometric EIA Kit
Application: ELISA
Species reactivity: human
1,061.00€ *
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Bovine IgG ELISA Kit
Bovine IgG ELISA Kit

Item number: E11-118

Sandwich ELISA. Complete kit for quantitating Bovine IgG levels in serum, plasma, milk and colostrum. For up to 40 samples, tested in duplicate.
Keywords: EIA
Application: ELISA
Species reactivity: bovine
850.00€ *
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Human Inflammatory Cytokine multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, M
Human Inflammatory Cytokine multiplex ELISA Kit (IL1...

Item number: ARG80929.96

Inflammation is body's protective response to foreign subjects, pathogens, tissue damage, autoimmune and other harmful stimuli. It is initiated by production of a cascade of chemicals and cytokines in affected area. These pro-inflammatory mediators result in vasodilation, increased vascular permeability, influx of...
Keywords: IL1a, IL1b, IL6, IL8, CSF2, INFg, MCAF, TNFa
Application: ELISA
Species reactivity: human
2,392.00€ *
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DNA/RNA Oxidative Damage Express ELISA Kit
DNA/RNA Oxidative Damage Express ELISA Kit

Item number: Cay502890-96

Cayman Chemical DNA/RNA Oxidative Damage Express Kit detects all three oxidized guanine/guanosine species. 8-OHdG from DNA, 8-OHG from RNA, and 8-hydroxyguanine from either DNA or RNA. Some commercial vendors offer immunoassays that detect only 8-OHdG, and not the other two molecules. The advantage of the Cayman kit...
Keywords: 8-hydroxy-2'-Deoxyguanosine, 8-Hydroxyguanosine, 8-OHdG, 8-OHG, Deoxyribonucleic Acid, Ribonucleic Acid, Biomarker DNA...
Application: Competitive ELISA
927.00€ *
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Cyclic CMP ELISA Kit
Cyclic CMP ELISA Kit

Item number: Cay502480-96

Cayman's Cyclic CMP ELISA Kit is a competitive assay that can be used for the quantification of cCMP in bacterial lysates. The assay has a range of 0.156-20 nM, an average sensitivity (80% B/B0) of 0. nM, and a lower limit of detection (LLOD) of 0. nM.
Keywords: Cyclic Cytidine 3'5'-monophosphate, cCMP
Application: ELISA
681.00€ *
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Alternative Complement Pathway, Mouse, Assay
Alternative Complement Pathway, Mouse, Assay

Item number: HYC-HIT422

The HIT422 measures the alternative pathway activity mediated via LPS. Complement deficiencies or other defects in the complement system can easily be screened by running an assay for each pathway in parallel or separately. The ELISA contains a positive control which can be used as control to ensure that the...
Keywords: AP, alternative pathway
Application: ELISA
Species reactivity: mouse
929.00€ *
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Complement Pathway, Mouse, Assay
Complement Pathway, Mouse, Assay

Item number: HYC-HIT423

The HIT423 contains three Complement Pathway Assays: - HIT420, Classical Complement Pathway, Mouse - HIT421, Lectin Complement Pathway, Mouse - HIT422, Alternative Complement Pathway, Mouse The innate immune system is important in the first defense against foreign pathogens. A major component of this response is the...
Keywords: Complement Pathway
Application: ELISA
Species reactivity: mouse
1,854.00€ *
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TCC, Rat, ELISA kit
TCC, Rat, ELISA kit

Item number: HYC-HK106-01

Protein Function: Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:9212048). The MAC is initiated by proteolytic cleavage...
Keywords: C9, Complement component C9
Application: ELISA
Species reactivity: rat
From 858.00€ *
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MASP-1/C1-INH complex, Human, ELISA kit
MASP-1/C1-INH complex, Human, ELISA kit

Item number: HYC-HK3001-01

Protein Function: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to...
Keywords: MASP1, Serine protease 5, Complement factor MASP-3, Ra-reactive factor serine protease p100, Mannan-binding lectin serine...
Application: ELISA
Species reactivity: human
From 929.00€ *
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FHR-4, Human, ELISA kit
FHR-4, Human, ELISA kit

Item number: HYC-HK3006-01

The FHR-4, human, ELISA kit is to be used for the in vitro quantitative determination of FHR-4 in serum and plasma samples.
Keywords: CFHR4, Complement factor H-related protein 4
Application: ELISA
Species reactivity: human
From 858.00€ *
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BPI, Human, ELISA kit
BPI, Human, ELISA kit

Item number: HYC-HK314-01

Protein Function: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria, this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial...
Keywords: BPI, CAP 57, Bactericidal permeability-increasing protein
Application: ELISA
Species reactivity: human
From 715.00€ *
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LL-37, Human, ELISA kit
LL-37, Human, ELISA kit

Item number: HYC-HK321-01

Protein Function: Antimicrobial protein that is an integral component of the innate immune system (PubMed:14978112, PubMed:16637646, PubMed:18818205, PubMed:22879591, PubMed:9736536). Binds to bacterial lipopolysaccharides (LPS) (PubMed:16637646, PubMed:18818205). Acts via neutrophil N-formyl peptide receptors to...
Keywords: CAMP, Cathelicidin antimicrobial peptide, 18 kDa cationic antimicrobial protein
Application: ELISA
Species reactivity: human
From 858.00€ *
Review
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