ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Interleukin-1beta (human) ELISA Kit
Interleukin-1beta (human) ELISA Kit

Item number: Cay583311-96

Interleukin 1 (IL-1) is a name for a family of proteins which includes IL-1alpha, IL-1beta, ILra (Interleukin receptor antagonist) and IL-18. IL-1beta is synthesized as 269 amino acid pro-cytokine that is enzymatically cleaved by IL-1beta-converting enzyme (ICE) into the active 154 amino acid, 17 kDa peptide mature...
Keywords: IL-1beta (human) EIA Kit
Application: ELISA
Species reactivity: human
635.00€ *
Review
Periostin (human) ELISA Kit
Periostin (human) ELISA Kit

Item number: AG-45B-0004-KI01

Protein function: Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX By similarity. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. [The UniProt...
Keywords: PN, OSF2, POSTN, OSF-2, Periostin, Osteoblast-specific factor 2
Application: ELISA
Species reactivity: human
627.00€ *
Review
SARS-CoV-2 Spike RBD IgG (mouse) ELISA Kit
SARS-CoV-2 Spike RBD IgG (mouse) ELISA Kit

Item number: Cay502820-96

Cayman's SARS-CoV-2 Spike RBD IgG (mouse) ELISA Kit can be used to assess the presence of antibodies against the SARS-CoV-2 spike RBD (Spike RBD) in mouse plasma and serum. Cayman's SARS-CoV-2 Spike RBD IgG (mouse) ELISA Kit does not differentiate between neutralizing and non-neutralizing antibodies, including those...
Keywords: Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Spike Glycoprotein Receptor Binding Domain IgG
Application: Serological assay
882.00€ *
Review
Human BD-2 / beta Defensin-2 ELISA kit
Human BD-2 / beta Defensin-2 ELISA kit

Item number: ARG80903.96

Protein function: Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed:20068036). [The UniProt Consortium]
Keywords: Anti-BD-2, Anti-SAP1, Anti-hBD-2, Anti-DEFB4B, Anti-DEFB4A, Anti-DEFB102, Anti-Beta-defensin 2, Anti-Beta-defensin 4A,...
Application: ELISA
Species reactivity: human
678.00€ *
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Classical Complement Pathway, Rat, Assay
Classical Complement Pathway, Rat, Assay

Item number: HYC-HIT410

The HIT410 measures the classical pathway activity mediated via IgM. Complement deficiencies or other defects in the complement system can easily be screened by running an assay for each pathway in parallel or separately. The ELISA contains a positive control based on the Wistar strain which can be used as process...
Keywords: CP
Application: ELISA
Species reactivity: rat
929.00€ *
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Classical Complement Pathway, Mouse, Assay
Classical Complement Pathway, Mouse, Assay

Item number: HYC-HIT420

The HIT420 measures the classical pathway activity mediated via IgM. Complement deficiencies or other defects in the complement system can easily be screened by running an assay for each pathway in parallel or separately. The ELISA contains a positive control which can be used as process control to ensure that the...
Keywords: CP
Application: ELISA
Species reactivity: mouse
929.00€ *
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C3c, Human, ELISA kit
C3c, Human, ELISA kit

Item number: HYC-HK368-01

Protein Function: Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system , Non-enzymatic component of C5...
Keywords: C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
Application: ELISA
Species reactivity: human
From 858.00€ *
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Unacylated Ghrelin (human) Express EIA kit
Unacylated Ghrelin (human) Express EIA kit

Item number: BR-A05119.96

Protein function: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. [The UniProt Consortium]
Keywords: GHRL, MTLRP
Species reactivity: human
688.00€ *
Review
Human IL4I1 (Interleukin 4 Induced Protein 1) ELISA Kit
Human IL4I1 (Interleukin 4 Induced Protein 1) ELISA Kit

Item number: G-HUES03170.96

ELISA Type: Sandwich. Sensitivity: 0.094ng/mL. Range: 0.156--10ng/mL. Protein function: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. [The UniProt Consortium]
Keywords: LAO, LAAO, FIG1, IL4I1, hFIG1, EC=1.4.3.2, Protein Fig-1, L-amino-acid oxidase, IL4-induced protein 1,...
Application: Sandwich ELISA
Species reactivity: human
708.00€ *
Review
Human Therapeutic IgG4 ELISA Kit
Human Therapeutic IgG4 ELISA Kit

Item number: Cay500920-96

Cayman's Human Therapeutic IgG4 ELISA Kit is an immunometric (i.e. sandwich) assay that can be used for the quantification of human IgG4 in monkey, rodent, or rabbit plasma and serum without prior sample purification. The standard curve spans the range of 7.8-500 ng/ml, with a lower limit of quantification (LLOQ) of...
Keywords: Human IgG4 Immunometric EIA Kit
Application: ELISA
Species reactivity: human
1,093.00€ *
Review
Bovine IgG ELISA Kit
Bovine IgG ELISA Kit

Item number: E11-118

Sandwich ELISA. Complete kit for quantitating Bovine IgG levels in serum, plasma, milk and colostrum. For up to 40 samples, tested in duplicate.
Keywords: EIA
Application: ELISA
Species reactivity: bovine
From 850.00€ *
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DNA/RNA Oxidative Damage Express ELISA Kit
DNA/RNA Oxidative Damage Express ELISA Kit

Item number: Cay502890-96

Cayman Chemical DNA/RNA Oxidative Damage Express Kit detects all three oxidized guanine/guanosine species. 8-OHdG from DNA, 8-OHG from RNA, and 8-hydroxyguanine from either DNA or RNA. Some commercial vendors offer immunoassays that detect only 8-OHdG, and not the other two molecules. The advantage of the Cayman kit...
Keywords: 8-hydroxy-2'-Deoxyguanosine, 8-Hydroxyguanosine, 8-OHdG, 8-OHG, Deoxyribonucleic Acid, Ribonucleic Acid, Biomarker DNA...
Application: Competitive ELISA
956.00€ *
Review
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