ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Mouse IFN-gamma (Interferon Gamma) ELISPOT Kit
Mouse IFN-gamma (Interferon Gamma) ELISPOT Kit

Item number: E-ESP-M0001.480

Protein function: Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:11585387, PubMed:8456301). Primarily signals through the JAK-STAT...
Keywords: Ifng, IFN-gamma, Interferon gamma, Mouse IFN-gamma (Interferon Gamma) ELISPOT Kit
Application: ELISPOT
From 426.00€ *
Review
Human IL-5 (Interleukin 5) ELISPOT Kit
Human IL-5 (Interleukin 5) ELISPOT Kit

Item number: E-ESP-H0008.480

Protein function: Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and...
Keywords: IL5, TRF, IL-5, Interleukin-5, T-cell replacing factor, B-cell differentiation factor I, Eosinophil differentiation...
Application: ELISPOT
From 426.00€ *
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Human IL-6 (Interleukin 6) ELISPOT Kit
Human IL-6 (Interleukin 6) ELISPOT Kit

Item number: E-ESP-H0009.480

Protein function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and...
Keywords: CDF, IL-6, IFNB2, BSF-2, IFN-beta-2, Interleukin-6, Interferon beta-2, Hybridoma growth factor, CTL differentiation...
Application: ELISPOT
From 426.00€ *
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Human IL-10 (Interleukin 10) ELISPOT Kit
Human IL-10 (Interleukin 10) ELISPOT Kit

Item number: E-ESP-H0003.480

Protein function: Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and...
Keywords: CSIF, IL10, IL-10, Interleukin-10, Cytokine synthesis inhibitory factor, Human IL-10 (Interleukin 10) ELISPOT Kit
Application: ELISPOT
From 426.00€ *
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Human IL-2 (Interleukin 2) ELISPOT Kit
Human IL-2 (Interleukin 2) ELISPOT Kit

Item number: E-ESP-H0006.480

Protein function: Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535). Binds to a receptor complex composed of either the high-affinity trimeric...
Keywords: IL2, IL-2, TCGF, Aldesleukin, Interleukin-2, T-cell growth factor, Human IL-2 (Interleukin 2) ELISPOT Kit
Application: ELISPOT
From 426.00€ *
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Human IL-4 (Interleukin 4) ELISPOT Kit
Human IL-4 (Interleukin 4) ELISPOT Kit

Item number: E-ESP-H0007.480

Protein function: Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on...
Keywords: IL4, IL-4, BSF-1, Pitrakinra, Binetrakin, Interleukin-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1,...
Application: ELISPOT
From 426.00€ *
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7395 from 6872 pages