5-MTHF (5-Methyltetrahydrofolate) BioAssay(TM) ELISA Kit (Rat)

Sample Type:, Serum, plasma, tissue homogenates and other biological fluids. Intended Use: Competitive ELISA, Coated with Antigen, Sensitivity: <0.188ng/ml, Range: 0.313-20ng/ml, Specificity: Rat, Test Principle: This BioAssay(TM) ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 5-MTHF. During the reaction, 5-MTHF in the sample or standard competes with a fixed amount of 5-MTHF on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 5-MTHF. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of 5-MTHF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: Micro ELISA Plate (Dismountable), 8x12, Lyophilized Standard, 2xvial, Sample/Standard dilution buffer, 20ml, Biotin-detection antibody (Concentrated), 60ul, Antibody dilution buffer, 10ml, HRP-Streptavidin Conjugate (SABC), 120ul, SABC dilution buffer, 10ml, TMB substrate, 10ml, Stop solution, 10ml, Wash buffer (25X), 30ml, Plate Sealer, 5xpieces, Product Description, 1xcopy, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.