TSLPR:TSLP [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The TSLPR:TSLP [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between TSLPR (thymic stromal lymphopoietin receptor) and TSLP. This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled TSLP (amino acids 29-159), human TSLP-R (amino acids 25-231), streptavidin-HRP, and assay buffer for 100 reactions. A 96-well plate  is coated with TSLPR protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-TSLP, the plate is washed and Streptavidin-HRP is added. The ELISA ECL substrate is added  and the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of TSLPR to TSLP.