Transcreener(R) dAMP FP Assay

The Transcreener(R) dAMP FP Assay is a far-red, competitive fluorescence polarization (FP) assay. The assay is designed to be used with exonuclease enzymes such as TREX1 (also known as Three Prime Repair Exonuclease 1), that produce the product dAMP (deoxyadenosine monophosphate, deoxyadenylic acid, or deoxyadenylate) by cleaving sources of DNA. An example is the human enzyme TREX1 which is the major exonuclease responsible for degrading cytosolic DNA. The Transcreener assay is designed specifically for high throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The Transcreener dAMP FP Assay provides the following benefits:  A simple single addition exonuclease activity assay capable of HTS.  Excellent data quality (Z' > 0.7) and signal (>85 mP polarization shift) at dAMP ranges between 0.110 µM.  Sensitive detection of dAMP down to 10 nM and as high as 100 µM.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.