Transcreener(R) AMP2/GMP2 TR-FRET Assay

The Transcreener(R) AMP2/GMP2 TR-FRET Red Assay is a competitive immunoassay for enzymes that produce AMP or GMP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay relies on a highly specific monoclonal antibody that recognizes AMP or GMP with more than 1,000-fold selectivity over substrate nucleotides, including ATP, cAMP, or cGMP. Enzymes that can be used with the assay include ubiquitin, SUMO, nucleic acid and protein ligases, phosphodiesterases (PDEs), and synthetases. ¶The Transcreener assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. ¶The Transcreener AMP2/GMP2 TR-FRET Red Assay provides the following benefits:  Accommodates substrate concentrations ranging from 0.1 µM to 1,000 µM.  Excellent data quality (Z' > 0.7) at low substrate conversion (typically 10-30%).  The only assay method that allows detection of unmodified AMP and GMP without using coupling enzymes.  Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.  Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.