Transcreener(R) ADPR FP Assay

The Transcreener(R) ADPR FP Assay is a biochemical HTS assay for measuring the production of ADP-ribose (ADPR) in enzymatic reactions. The assay uses a coupling enzyme to convert ADPR into AMP, which is then detected using a far-red, competitive fluorescence polarization (FP) assay. As examples, the Transcreener ADPR FP assay can be used to detect the activity of human CD38, which forms ADPR from the hydrolysis of NAD, or Poly (ADP-ribose) glycohydrolase (PARG), which produces ADPR from the breakdown of poly-ADP-ribose. The Transcreener ADPR FP assay is designed specifically for high throughput screening (HTS), with a single-addition, mix-and-read format. It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), sensitive detection of ADPR down to 10 nM and as high as 50 µM, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z' > 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. Key Applications:  Screening for enzyme inhibitors/activators  Generating dose response curves and IC 50 values for inhibitors.  Kinetic and mechanistic analyses.