Transcreener(R) ADO CD73 FP Assay

The Transcreener(R) CD73 FP Assay is a far-red, competitive fluorescence polarization (FP) assay. The assay is designed to be used with enzymes such as ecto-5'-nucleotidase (also known as 5'-nucleotidase, NT5E, Cluster of Diferentiation 73, or CD73), that produce the product adenosine (ADO) The Transcreener CD73 FP Assay is simple biochemical assay for measuring CD73 activity based on the Transcreener ADP2 Assay. The assay uses a coupling enzyme to convert adenosine into AMP and ADP in the presence of ATP. The Transcreener assay is designed specifically for high throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The Transcreener CD73 FP Assay provides the following benefits:  A simple single addition CD73 activity assay capable of HTS.  Excellent data quality (Z' > 0.7) and signal (>85 mP polarization shift) at ADO ranges between 0.3 µM and 3 µM.  Far-red tracer further minimizes interference from fluorescent compounds and light scattering.