Total Protein (TP) Colorimetric Assay Kit (Coomassie Brilliant Blue Method)

Detection principle Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm. Performance characteristics Synonyms TP Sample type Serum,plasma,animal tissue Sensitivity 0.026 mg/mL Detection range 0.026-1.2 mg/mL Detection method Colorimetric method Assay type Quantitative Assay time 50 min Precision Average inter-assay CV: 2.3%Average intra-assay CV: 1.8% Other instruments required Micropipettor, Vortex mixer Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) Storage Reagent 1: 2-8 °C, Reagent 2: RT Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg/mL). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor Human serum 40-60 10% Mouse liver tissue homogenization 8-12 Rat serum 40-60 Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).