TL1A: DR3 Inhibitor Screening TR-FRET Assay Kit

The TL1A: DR3 Inhibitor Screening TR-FRET Assay Kit is designed for the screening and profiling of neutralizing antibodies or inhibitors of the interaction between TL1A (TNF-like ligand 1A) and DR3 (Death Receptor 3) using TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer). This kit requires no time-consuming washing steps and comes in a convenient 384-well format, with purified biotinylated-DR3 (amino acids 25-199), europium-labeled (Eu) TL1A (amino acids 72-end), Dye-labeled Acceptor, and assay buffer for 400 reactions. Figure 1. Illustration of the mechanism of the TL1A: DR3 Inhibitor Screening TR-FRET Assay Kit.A sample containing europium-labeled TL1A, a dye-labeled streptavidin acceptor, biotinylated DR3, and an inhibitor, is incubated for 30 minutes. The europium-labeled TL1A binds to DR3-biotin, which receives the dye-labeled streptavidin acceptor. The TR-FRET signal is generated by proximity induced upon interaction of TL1A with DR3. Then, the fluorescence intensity is measured using a fluorescence reader capable of TR-FRET measurements. The signal generated is proportional to TL1A and DR3 binding activity.