StrandBrite(TM) Green Fluorimetric RNA Quantitation Kit *Optimized for Microplate Readers*

Ex: 500 nm. Em: 525 nm. Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm. The major disadvantage of the absorbance-based method is the interferences caused by proteins, free nucleotides and other UV absorbing compounds. The use of sensitive, fluorescent nucleic acid stains alleviates this interference problem. StrandBrite(TM) RNA quantifying reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. StrandBrite(TM) RNA quantifying reagent can detect as little as 5 ng/mL RNA with a fluorescence microplate reader or fluorometer. Our StrandBrite(TM) Green Fluorimetric RNA Quantitation Kit includes our StrandBrite(TM) Green nucleic acid stain with an optimized and robust protocol. It provides a convenient method for quantifying RNA in solutions.