S-Nitrosylated Protein Detection Kit (Biotin Switch)

S-Nitrosylated Protein Detection Kit (Biotin Switch)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10006518-1 1 each -

6 - 10 business days*

332.00€
 
Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions... more
Product information "S-Nitrosylated Protein Detection Kit (Biotin Switch)"
Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology. NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS). Cayman's S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-NO proteins in whole cells or tissues, as well as by western blot analysis. Using this method, free SH groups are first blocked (an addition of 125.1 amu per residue) and any S-NO bonds present in the sample are then cleaved. Biotinylation of the newly formed SH groups (an addition of 523.6 amu per residue) provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Synonyms: S-NO. Origin: Plant/Armoracia rusticana.
Keywords: S-NO
Supplier: Cayman Chemical
Supplier-Nr: 10006518

Properties

Application: S-nitrosylated protein detection, WB
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H300, H312, H314, H315, H317, H319, H332, H334, H335
P Phrases: P260, P261, P264, P270, P271, P272, P280, P284, P301+310, P301+330+331, P302+352, P303+361+353, P304+340, P304+341, P305+351+338, P310, P312, P321, P330, P332+313, P333+313, P337+313, P342+311, P361+364, P362+364, P363, P403+233, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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