Pyruvate Decarboxylase (PDC) Activity Assay Kit

Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm. Performance characteristics Synonyms: PDC Sample type: serum, plasma, tissue and cell samples Sensitivity: 0.67 U/L Detection range: 0.67-27.73 U/L Detection method: Colorimetric method Assay type: Enzyme Activity Assay time: 15 min Precision: Average inter-assay CV: 3.100%Average intra-assay CV: 2.800% Other instruments required: Vortex mixer, Centrifuge Other reagents required: Normal saline (0.9% NaCl)