Protein Carbonyl Colorimetric Assay Kit (Tissue And Serum Samples)

Detection principle The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly. Performance characteristics Sample type serum,plasma,hydrothorax,cell culture supernatant,tissue Detection method Colorimetric method Assay type Quantitative Assay time 140 min Precision Average inter-assay CV: 8.5%Average intra-assay CV: 5.2% Other instruments required Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge Other reagents required Absolute ethanol, Ethyl acetate Storage 2-8 °C Valid period 6 months