PRMT6, Chemiluminescent, BioAssay(TM) Kit (Protein Arginine N-Methyltransferase 6, Histone-Arginine

PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3. Intended Use: The PRMT6 Direct Activity BioAssay(TM) kit is designed to measure PRMT6 activity for screening and profiling applications. Test Principle: The key to the PRMT6 Direct Activity BioAssay(TM) Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt, Applications: Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications. Kit Components: Plate precoated with histone substrate, 1x96 wells, PRMT6 human recombinant enzyme, 1x20ug, 20uM S-adenosylmethionine*, 1x250ul , Primary antibody 4, 1x100ul , Secondary HRP-labeled antibody 2, 1x10ul , 4x HMT assay buffer 6, 1x3ml , Blocking buffer, 1x50ml , HRP chemiluminescent substrate A, (transparent bottle), 1x6ml , HRP chemiluminescent substrate B, (brown bottle), 1x6ml , *Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the, inhibitors. Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.