Portelite(TM) Rapid Fluorimetric Biotin Quantitation Kit *Optimized to Use with CytoCite(TM) and Qub

Portelite(TM) Rapid Fluorimetric Biotin Quantitation Kit is specifically optimized to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite(TM) and Qubit(TM) Fluorometer. The kit uses Biotinylite(TM) Green, a fluorogenic biotin sensor. Biotinylite(TM) Green is almost non-fluorescent and give strong green fluorescence upon interaction with a biotin or biotin conjugate. The concentration of biotin is proportional to the fluorescence intensity of Biotinylite(TM) Green. The amount of biotin is determined by comparing a sample's fluorescence to the predetermined biotin standard curve. This fluorescence-based assay is much more sensitive than the commonly used colorimetric HABA assay. Biotin is a relatively small molecule that is routinely conjugated to antibodies and proteins with minimal interference of their biological activity. The avidin/streptavidin-biotin interaction is the strongest known binding pair between a protein and its ligand. The biotin-avidin interaction has been extensively explored for a variety of biological applications. Platform: Qubit Fluorometer, Excitation 490 nm, Emission 530 nm, Instrument specification(s): Green Fluorescent Channel. Components: Component A: Biotinylite(TM) Green (10X) 2 Vials (0.5 mL/Vial), Component B: Biotin Standard (300 µM) 2 Vials (100 µL/Vial), Component C: Assay Buffer 1 Bottle (25 mL).