Portelite(TM) Fluorimetric Protein Quantitation Kit *Optimized for CytoCite(TM) and Qubit(TM) Fluoro

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications. Biuret, Lowry, BCA and Bradford assays are routinely used for estimating protein concentration. However, these colorimetric assays are less sensitive, and require large sample volume to ensure accuracy. Our Portelite(TM) Fluorimetric Protein Quantitation Kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays. Prolite(TM) Orange used in the kit is non-fluorescent in aqueous solution, but reacts rapidly with proteins and generates bright fluorescence. The Portelite(TM) Fluorimetric Protein Quantitation Kit provides a simple method for quantifying protein concentration in solutions. The assay has dynamic range from 12.5 ug/mL to 5 mg/mL of BSA. The kit is optimized for Cytocite(TM) and Qubit(TM) fluorometers. It can be used for (1) studying protein/protein interactions, (2) measuring column fractions after affinity chromatography, (3) estimating recovery of membrane proteins from cell extract, and (4) high-throughput screening of fusion proteins.