NMT1 Fluorogenic Assay Kit

The NMT1 Fluorogenic Assay Kit is designed to measure NMT1 (N-myristoyltransferase 1) N-myristoylation activity for screening and profiling applications. The kit comes in a convenient 96-well format, with purified recombinant NMT1 (amino acids 110-496), NMT Peptide 1, Myristoyl - coenzyme A (MCoA), CPM (fluorescent dye), and assay buffer for 100 enzyme reactions. Figure 1. Mechanism of action of NMT1 Fluorogenic Assay Kit. NMT1 (N-myristoyltransferase 1), also known as Glycylpeptide N-tetradecanoyltransferase 1, is a member of the NMT family of proteins. It is a cytosolic enzyme that catalyzes the addition of a myristoyl group to glycine residues located in the N-terminus of eukaryotic, fungal and viral proteins. The addition of the myristoyl group allows for proper protein localization, and for cell survival and proliferation. It plays a role in embryonic development and cancer cell metabolism and growth. It is linked to breast, colon, and other cancer types, contributing to drug resistance. Research has found that inhibitors for NMT1 can lead to tumor growth reduction. Further studies into the role of this protein will open new treatment opportunities in cancer therapy.