MycoLight(TM) Fluorescence Live/Dead Bacterial Imaging Kit

AAT Bioquest's MycoLight(TM) Fluorescence Live/Dead Bacterial Imaging Kit provides two-color fluorescence assay for visualizing live and dead bacteria through fluorescent microscope. MycoLight(TM) 520 is a non-fluorescent esterase substrate that diffuses into both Gram positive and Gram-negative bacteria. Upon hydrolysis by bacterial intracellular non-specific esterases, a green fluorescent product is produced and accumulated within bacteria. In contrast, propidium iodide is a red-fluorescent nucleic acid stain that only penetrates bacteria with damaged membranes. Thus, with an appropriate mixture of the MycoLight(TM) 520 and propidium iodide stains, live bacteria with intact cell membranes emits green fluorescence, whereas dead bacteria with damaged membranes gives red fluorescence. The MycoLight(TM) Fluorescence Live/Dead Bacterial Imaging Kit is a robust tool for imaging Live/Dead bacteria. Stained cells can be monitored fluorimeterically (FITC filter set) and (TRITC filter set) for live and dead bacteria respectively.