Molecular Glue/PROTAC(R) Optimization Kit for RBM39-DCAF15

The Molecular Glue/PROTAC(R) Optimization Kit for RBM39-DCAF15 Binding is designed for the testing and profiling of Molecular Glues (MG)/PROTACs targeting the RBM39 (RNA-binding protein 39) and DCAF15 (DDB1 and CUL4 associated factor 15) interaction. The Molecular Glue/PROTAC(R) Optimization Kit for RBM39-DCAF15 Binding comes in a convenient AlphaLISA(R) format, with enough optimized assay and detection buffer, purified recombinant RBM39 (amino acids 245-530) and Rbx1/CUL4B/DDB1/DCAF15/DDA1 complex for 384 reactions. This kit also contains the molecular glue E7820 and Arginine as controls. Figure 1. Schematic representation of the Molecular Glue/PROTAC(R) Optimization Kit for RBM39-DCAF15 Binding assay kit principle.The Molecular Glue/PROTAC(R) of interest is incubated with DCAF15 complex and RBM39, bringing them in close proximity. DCAF15 contains a His-tag, which is recognized by the donor beads. RBM39 contains a GST tag that binds to the GSH- AlphaLISA(TM) acceptor beads. Upon excitation of the donor bead, a singlet oxygen is generated by the bead. The singlet oxygen excites the acceptor bead, which emits light proportionally to the level of interaction between RBM39 and DCAF15