MLL4 (KMT2B): WDR5 Binding Chemiluminescent Assay Kit

The MLL4 (KMT2B): WDR5 Binding Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between MLL4 (mixed lineage leukemia protein-4, also known as KMT2B) and WDR5 (WD40 repeat-containing protein 5) for screening and profiling applications. The MLL4 (KMT2B): WDR5 Binding Chemiluminescent Assay comes with enough purified MLL4 (amino acids 2490-2715) and WDR5 proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with MLL4 protein. After coating and blocking, WDR5 is added in an optimized assay buffer. Next, unbound WDR5 is washed away, and the plate is incubated with a primary antibody followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to MLL4 (KMT2B).