Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)

Detection principle MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm. Performance characteristics Synonyms: MDA Sample type: Cells Sensitivity: 0.29 nmol/mL Detection range: 0.29-100 nmol/mL Detection method: Colorimetric method Assay type: Quantitative Assay time: 65 min Precision: Average inter-assay CV: 3.5%Average intra-assay CV: 3.3% Other instruments required: Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer Other reagents required: Absolute ethanol Storage: 2-8? Valid period: 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor A549 cells 1 HepG2 cells 1 293T cells 1 Note: The diluent is reagent 5 working solution.